Pressing all 3 yellow buttons throughout the floor unlocks the bridge to Knife Piece 2. Yellow Buttons only appear in the second Mines or Ashpit floor if the player has acquired Knife Piece 1, and are square-shaped. Red Buttons often appear either hidden behind tall blocks or in rooms filled with enemies either too great in numbers to fight off normally or that are blocked from the player's attacks, therefore requiring the button to clear the room. Red Buttons also disarm all the same hazards that silver buttons can, but unlike said button red buttons don't need to be pressed to clear a room. Enemies that normally spawn another enemy or shoot projectiles upon death will not do so if slain by a Red Button. Once pressed, all enemies will be killed and all enemy projectiles will be destroyed. Red Buttons usually appear in normal rooms. This can be fixed by leaving the room and re-entering.

In trap rooms with retracting spikes, pressing the silver button within five frames of the spikes deploying will cause them to appear to fail to retract, but this is only a visual bug, and they will not deal damage. Unlike Reward Plates, all Silver Buttons in the room have to be pressed to clear the room even if all enemies are slain.

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